Composite
NLuc

Part:BBa_K4658004:Design

Designed by: Farouk Ghanem, Sonja Klose   Group: iGEM23_Stockholm   (2023-10-07)


NanoLuc luciferase fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 232
    Illegal NotI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 207
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The major design considerations were the positions of start and stop codon with respect to the His-tag on the backbone, and the restriction enzymes required.


Source

This sequence has been designed in the lab and does not come from a genomic sequence. Although, it consists of different parts that come from various genomic sources.

References

Kristin Rübsam, Benjamin Stomps, Alexander Böker, Felix Jakob, Ulrich Schwaneberg, Anchor peptides: A green and versatile method for polypropylene functionalization, Polymer, Volume 116, 2017, Pages 124-132, ISSN 0032-3861

Ji, Y., Lu, Y., Puetz, H., & Schwaneberg, U. (2021). Anchor peptides promote degradation of mixed plastics for recycling. In Methods in Enzymology (Vol. 648, pp. 271-292). Academic Press.